DeadEnd™ Fluorometric TUNEL System

TUNEL assay cell type - HeLa cells human cervical cancer

Experiment
TUNEL assay cell type - HeLa cells human cervical cancer
Product
DeadEnd™ Fluorometric TUNEL System from Promega
Manufacturer
Promega

Protocol tips

Downstream tips
- When there was a little or poor staining, optimise permeabilization step
by adjusting incubation time with permeabilization agent.


- When there is a loss of tissue from slides, decrease
Proteinase K incubation time.

Publication protocol

We applied MTT assay to determine cell viability. The number of cells was counted every 24 h for 7 days. A TUNEL apoptosis detection kit (Promega) was used for DNA fragmentation fluorescence staining according to the manufacturer’s protocol. Positively stained, fluorescein-labelled cells were visualised and counted using a fluorescence microscope (Nikon Eclipse 80i, China).

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Reviews

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - HeLa cells human cervical cancer using DeadEnd™ Fluorometric TUNEL System from Promega.

Paper title
Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo.
Full paper
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Manufacturer protocol

Download the product protocol from Promega for DeadEnd™ Fluorometric TUNEL System below.

Download PDF Download manufacturer protocol

Videos

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